AIDS Kaposi sarcoma-derived cells produce and respond to interleukin 6

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.11.4068· OSTI ID:5393656

Miles, S A; Rezai, A R; Salazar-Gonzalez, J F; Meyden, M V; Stevens, R H; Mitsuyasu, R T; Martinez-Maza, O ^[1]; Logan, D M ^[2]; Taga, Tetsuya; Hirano, Toshio; Kishimoto, Tadamitsu ^[3]

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1. Univ. of California School of Medicine, Los Angeles (USA)
2. Ottawa Regional Cancer Centre, Ontario (Canada)
3. Osaka Univ. (Japan)

Cell lines derived from Kaposi sarcoma lesions of patients with AIDS (AIDS-KS cells) produce several cytokines, including an endothelial cell growth factor, interleukin 1{beta}, and basic fibroblast growth factor. Since exposure to human immunodeficiency virus increases interleukin 6 (IL-6) production in monocytes and endothelial cells produce IL-6, the authors examined IL-6 expression and response in AIDS-KS cell lines and IL-6 expression in AIDS Kaposi sarcoma tissue. The AIDS-KS cell lines (N521J and EKS3) secreted large amounts of immunoreactive and biologically active IL-6. The authors found both IL-6 and IL-6 receptor (IL-6-R) RNA by slot blot hybridization analysis of AIDS-KS cells. The IL-6-R was functional, as ({sup 3}H)thymidine incorporation by AIDS-KS cells increased significantly after exposure to human recombinant IL-6 (hrIL-6) at >10 units/ml. When AIDS-KS cells (EKS3) were exposed to IL-6 antisense oligonucleotide, cellular proliferation decreased by nearly two-thirds, with a corresponding decrease in the production of IL-6. These results show that both IL-6 and IL-6-R are produced by AIDS-KS cells and that IL-6 is required for optimal AIDS-KS cell proliferation, and they suggest that IL-6 is an autocrine growth factor for AIDS-KS cells.

• OSTI ID: 5393656
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:11; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AIDS
ANIMAL CELLS
ANIMAL TISSUES
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL PROLIFERATION
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA HYBRIDIZATION
ENDOTHELIUM
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
HYDROGEN COMPOUNDS
IMMUNE SYSTEM DISEASES
INFECTIOUS DISEASES
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOKINES
MATERIALS
MEMBRANE PROTEINS
MESSENGER-RNA
MITOGENS
MONOCYTES
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOGENESIS
PATIENTS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RECEPTORS
RECOMBINANT DNA
RIBOSIDES
RNA
SARCOMAS
SYNTHESIS
THYMIDINE
TISSUES
TRITIUM COMPOUNDS
TUMOR CELLS
VIRAL DISEASES