Recombinant interferon regulation of lung fibroblast growth

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5393255

Elias, J A; Freundlich, B; Rossman, M D; Gustilo, K; Jimenez, S A

The authors characterized the effect on human lung fibroblast proliferation of recombinant interferons (gamma (..gamma..), alpha/sub A/ (..cap alpha../sub A/), alpha/sub D/ (..cap alpha../sub D/) and beta (..beta..)). Quiescent and log phase fibroblasts were used. Proliferation was assessed using /sup 3/HTdr uptake and cell counts. ..gamma.. stimulated the /sup 3/HTdr uptake of quiescent cells. Maximal stimulation was seen between 100 and 500 IU/ml. Fibroblasts incubated in 100 IU/ml incorporated 160 +/- 9% as much /sup 3/HTdr as quiescent controls (p < .04). In contrast, ..gamma.. caused a dose dependent inhibition of the /sup 3/HTdr uptake of log phase cells. Fibroblasts incubated in 1000 IU/ml incorporated 41 +/- 9% as much /sup 3/HTdr as log phase controls (p < .02). Unlike ..gamma.., ..cap alpha../sub A/, ..cap alpha../sub D/ and ..beta.. did not stimulate the proliferation of quiescent cells. However, they did inhibit the proliferation of log phase cells. Fibroblasts incubated in 1000 IU/ml of ..cap alpha../sub A/, ..cap alpha../sub D/ and ..beta.. incorporated 64 +/- 10, 81 +/- 4 and 62 +/- 7% as much /sup 3/HTdr as log phase controls (p < .03 for all). ..gamma.., ..cap alpha../sub A/, ..cap alpha../sub D/ and ..beta.. did not stimulate fibroblast prostaglandin E (PGE) production and incubating fibroblasts with indomethacin did not reverse the inhibition of fibroblast proliferation caused by the interferons. Thus, (1) ..gamma.. can stimulate or inhibit fibroblast growth, (2) ..cap alpha../sub A/, ..cap alpha../sub D/ and ..beta.. only inhibit fibroblast growth, (3) the inhibition of fibroblast growth caused by ..gamma.., ..cap alpha../sub A/, ..cap alpha../sub D/ and ..beta.. is largely independent of fibroblast PGE production.

• Research Organization: Univ. of Pennsylvania, Philadelphia
• OSTI ID: 5393255
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BODY
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
FIBROBLASTS
GROWTH
GROWTH FACTORS
INTERFERON
LABELLED COMPOUNDS
LUNGS
LYMPHOKINES
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROSTAGLANDINS
PROTEINS
RESPIRATORY SYSTEM
SOMATIC CELLS
TRITIUM COMPOUNDS