The labeling of pulmonary surfactant phosphatidylcholine in newborn and adult sheep

Journal Article · Sat Aug 01 04:00:00 EDT 1981 · Exp. Lung Res.; (United States)

DOI:https://doi.org/10.3109/01902148109052315· OSTI ID:5393034

Ikegami, M; Jobe, A; Nathanielsz, P W

The labeling of the saturated phosphatidylcholine from surfactant with radiolabeled palmitic acid was characterized in seven newborn and seven adult sheep using a repetitive sampling technique. Each animal had a small cannula placed surgically in the trachea. Following the intravenous injection of (3H) palmitic acid, surfactant samples in saline were recovered from the distal airways of each animal with fine plastic catheters over a period of 10 days. The change in specific activity of the saturated phosphatidylcholine (cpm/mumol) was used to define the kinetics of secretion and then disappearance of the labeled saturated phosphatidylcholine. Labeled saturated phosphatidylcholine accumulated in a linear fashion without an apparent initial delay for 27 hr in adult and 44 hr in newborn sheep. The labeled saturated phosphatidylcholine then decayed with mean apparent biological half-life values of 45 hr and 54 hr in adult and newborn sheep, respectively. However, these half-life estimates are compromised by the long secretory phase of the labeling curves. The characteristics of the labeling of surfactant saturated phosphatidylcholine in sheep may be more representative of surfactant metabolism in large mammals than previous studies in small rodents.

• Research Organization: Department of Pediatrics, UCLA Medical Center, Torrance
• OSTI ID: 5393034
• Journal Information: Exp. Lung Res.; (United States), Journal Name: Exp. Lung Res.; (United States) Vol. 2:3; ISSN EXLRD
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CHEMICAL PREPARATION
DOMESTIC ANIMALS
ESTERS
HEXADECANOIC ACID
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LABELLING
LIPIDS
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
NEONATES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
REACTION KINETICS
RUMINANTS
SHEEP
SURFACTANTS
SYNTHESIS
TRITIUM COMPOUNDS
VERTEBRATES