Identification of a lymphocyte-produced cysteine proteinase based on its binding to. cap alpha. -macroglobulin (. cap alpha. M)

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5392992

Schlesinger, C; Hagen, K; Borth, W; Teodorescu, M

Proteinases play a role in collagen vascular diseases. However, the role of lymphocytes in the production of these proteinases is poorly understood. Rabbit lymph node cells (LNC) were cultured with /sup 35/S-methionine or /sup 14/C-leucine. When culture supernatants were analyzed by radioimmunoelectrophoresis using an anti-rabbit ..cap alpha..M antiserum, radio-active ..cap alpha..M-precipitin arcs developed. LNC biosynthetically labeled in serum-free medium generated supernatants that, upon incubation with normal rabbit serum, yielded radio-active ..cap alpha..M precipitin arcs. Purified B and T cells as well as human T and B cell lines were labeled separately under identical conditions. Analysis revealed the presence of radiolabeled ..cap alpha..M molecules. T cells usually generated less intense radioactive ..cap alpha..M precipitin arcs than B cells. Thoracic duct lymphocytes also produced radioactive ..cap alpha..M precipitin arcs. They treated radiolabeled supernatants with a variety of proteinase inhibitors before reacting them with rabbit serum. Only inhibitors specific for cysteine or thiol proteinases were effective in abolishing radioactivity associated with ..cap alpha..M. It appears that lymphocytes actively produce a cysteine or thiol proteinase which, theoretically, is capable of degrading collagen.

• Research Organization: Univ. of Illinois, Chicago
• OSTI ID: 5392992
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CULTURES
COLLAGEN
CONNECTIVE TISSUE CELLS
CYSTEINE
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
EVEN-ODD NUCLEI
GLOBULINS
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LEUCINE
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
METHIONINE
MOLECULES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDE HYDROLASES
PROTEINS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SCLEROPROTEINS
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
THIOLS
TRACER TECHNIQUES
VERTEBRATES