Interaction of human and chick DNA repair functions in UV-irradiated xeroderma pigmentosum-chick erythrocyte heterokaryons

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(82)90019-2· OSTI ID:5389475

Bootsma, D ^[1]; Keijzer, W; Vander Veer, E; Rainald, G; De Weerd-Kastelein, E A

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1. Erasmus Univ., Rotterdam, Netherlands

Fusion of chick erythrocytes with human primary fibroblasts results in the formation of heterokaryons in which the inactive chick nuclei become reactivated. The expression of chick DNA repair functions was investigated by the analysis of the DNA repair capacity after exposure to ultraviolet (UV) irradiation of such heterokaryons obtained after fusion of chick erythrocytes with normal human or xeroderma pigmentosum (XP) cells of complementation groups A, B, C and D. Unscheduled DNA synthesis (UDS) in normal human nuclei in these heterokaryons is suppressed during the first 2-4 days after fusion. The extent and duration of this suppression is positively correlated with the number of chick nuclei in the heterokaryons. Suppression is absent in heterokaryons obtained after fusion of chicken embryonic fibroblasts with XP cells (complementation group A and C). Restoration of DNA repair synthesis is found after fusion in XP nuclei of all complementation groups studied. It occurs rapidly in XP group A nuclei, starting one day after fusion and reaching near normal human levels after 5-8 days. In nuceli of the B, C and D group increased levels of UDS are found 5 days after fusion. At 8 days after fusion the UDS level is about 50% of that found in normal human nuclei. The pattern of UDS observed in the chick nuclei parallels that of the human counterpart in the fusion. In heterokaryons obtained after fusion of chick fibroblasts with XP group C cells UDS remains at the level of chick cells. These suggest that reactivation of chick erythrocyte nuclei results in expression of repair functions which are able to complement the defects in the XP complementation groups A, B, C and D.

• OSTI ID: 5389475
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 137:1; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

560114 -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BIOLOGICAL REPAIR
BIOSYNTHESIS
BIRDS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHICKENS
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ERYTHROCYTES
FIBROBLASTS
FOWL
HYBRIDIZATION
MAMMALS
MAN
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES
XP CELLS