Glucagon-stimulated ethanol metabolism in the liver of the rat

Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5387439

Bernstein, J; Escobales, N; Martinez, B

Rat liver slices incubated in a Krebs Ringer Bicarbonate (or TRIS) buffer metabolize ethanol at about 80-160 umoles/g x hr. Dinitrophenol (10 mM), an uncoupler of mitochondrial oxidative phosphorylation, stimulated ethanol metabolism by 75%, whereas cyanide (3 mM0, an inhibitor of mitochondrial oxidations, decreased it by about 50%. Glucagon (10..sqrt../sub 7/M) increased ethanol metabolism by about 100%. Glucagon-stimulated ethanol metabolism was inhibited by 10 mM Ouabain, an inhibitor of the sodium pump (from 11.66 +/- 1.37 to 6.83 +/- 0.75 ..mu..moles/g x 5 min), and by 10 ..mu..M Atractyloside, an inhibitor of ATP/ADP translocation across the inner mitochondrial membrane (from 10.64 +/- 2.32 to 6.06+/- 2.02 ..mu..moles/g x 5 min). Glucagon also increased the active transport of /sup 87/Rb (from 217.25 +/- 31.24 to 354.24 +/- 41.56 mmoles/Kg dry wt x hr) without changing passive transport of this ion. These results suggest that glucagon, by stimulating the activity of the sodium pump, increases the ability of mitochondria to reoxidize reducing equivalents generated during ethanol metabolism, thus accounting for at least one-half of the increased ethanol metabolism observed in the presence of this hormone.

• Research Organization: Univ. of Puerto Rico, San Juan
• OSTI ID: 5387439
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALI METAL ISOTOPES
ANIMALS
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CYANIDES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DINITROPHENOL
ETHANOL
GLANDS
GLUCAGON
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIVER
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
METABOLISM
MINUTES LIVING RADIOISOTOPES
MITOCHONDRIA
NITRO COMPOUNDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PHENOLS
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RATS
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
TRACER TECHNIQUES
VERTEBRATES