Autotrophic growth: methylated carbon monoxide dehydrogenase as an intermediate of acetyl-CoA synthesis

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5387339

Pezacka, E; Wood, H G

A new pathway of autotrophic growth has been discovered in certain anaerobic bacteria in which acetyl-CoA is the product formed from CO/sub 2/ for initiation of anabolism rather than 3-phosphoglycerate as in the Calvin Cycle. CO/sub 2/ is reduced in combination with tetrahydrofolate to methyltetrahydrofolate (CH/sub 3/THF) and is the source of the CH/sub 3/ group. CO/sub 2/ or CO is the source of the carbonyl group. CO dehydrogenase (CODH), corrinoid enzyme, methyltransferase, ferredoxin and CODH disulfide reductase have been isolated from Clostridium thermoaceticum and shown to catalyze the synthesis of acetyl-CoA from CH/sub 3/THF, CO and CoA. The methyltransferase catalyzes transfer of the CH/sub 3/ group from CH/sub 3/THF to the corrinoid enzyme from which the methyl is transferred to CODH. CO is bound to the Ni of CODH forming a Ni-Fe-C center. When CO/sub 2/ is the source of carbon, H/sub 2/ and hydrogenase are required for reduction of the CO/sub 2/ by CODH. CODH disulfide reductase is required for the addition of CoA to the CODH (Pezacka, E. and Wood, H.G. J. Biol. Chem., in press). Then, CODH catalyzes the combination of the three groups forming acetyl-CoA. The authors have now succeeded in methylating CODH using /sup 14/CH/sub 3/I or /sup 14/CH/sub 3/-B/sub 12/. With the resulting /sup 14/CH/sub 3/-CODH, only CODH disulfide reductase is required for synthesis of (/sup 14/C)acetyl-CoA from CO and CoA. The amino acid sequence at the CH/sub 3/-site is being investigated.

• Research Organization: Case Western Reserve Univ., Cleveland, OH
• OSTI ID: 5387339
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON MONOXIDE
CARBON OXIDES
CHALCOGENIDES
CLOSTRIDIUM
COENZYMES
ENZYME ACTIVITY
ENZYMES
GROWTH
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
SYNTHESIS
TRACER TECHNIQUES