Synthesis of angiotensins by cultured granuloma macrophages in murine schistosomiasis mansoni
Components of the angiotensin system are present in granulomas of murine schistosomiasis mansoni. Angiotensins may have immunoregulatory function. Granuloma macrophages cultured for up to 3 days generated substantial angiotensin I (AI) and angiotensin II (AII) which appeared in the culture supernatants. Macrophage monolayers were incubated with (/sup 3/H) amino acids, and culture supernatants were extracted with acetone and analyzed by HPLC. Radiolabeled products eluded at times corresponding to those of authentic angiotensins. Immunoadsorption of angiotensins with angiotensin antisera removed reputed radiolabeled angiotensins from the supernatants. Treatment of the elution fraction corresponding to that of authentic AI with angiotensin converting enzyme resulted in the generation of radiolabeled polypeptides which co-eluted with authentic AII and His-Leu. Similar experiments conducted with nonadherent granuloma cells devoid of macrophages failed to demonstrate angiotensin production. These results suggest that granuloma macrophages can synthesize angiotensin.
- Research Organization:
- Wayne State Univ., Detroit, MI
- OSTI ID:
- 5387007
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANGIOTENSIN
BIOSYNTHESIS
MACROPHAGES
IMMUNE REACTIONS
AMINO ACIDS
BIOLOGICAL FUNCTIONS
GRANULOMAS
LIQUID COLUMN CHROMATOGRAPHY
MICE
POLYPEPTIDES
SCHISTOSOMIASIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
CARBOXYLIC ACIDS
CARDIOVASCULAR AGENTS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
FUNCTIONS
GLOBULINS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITIC DISEASES
PEPTIDES
PHAGOCYTES
PROTEINS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
SYNTHESIS
VASOCONSTRICTORS
VERTEBRATES
550901* - Pathology- Tracer Techniques