Isolation and characterization of cryptic plasmids from filamentous, nonheterocystous cyanobacteria
Conference
·
· Plant Physiol.; (United States)
OSTI ID:5383688
Many filamentous, nonheterocystous, N/sub 2/-fixing cyanobacteria from the LPP group contain cryptic plasmids. Plasmid number and size were determined as well as sequence homologies among independent strains of the same species, and different species. Only a few studied have been carried out to determine plasmid profiles in filamentous non-heterocystous cyanobacteria. Also, sequence homologies between plasmids have been examined only in the unicellular organisms. Using both liquid and solid BG-11 culture media, various strain specific, rapid, small-scale plasmid isolation procedures have been developed. These include lysozyme/SDS incubations, phenol and chloroform extractions, and an isopropanol precipitation. Resuspended pellets were electrophoresed on agarose gels, providing a quick and accurate method of determining plasmid size and number. Several endogenous plasmids have been isolated, cloned, and mapped through restriction enzyme digestion. These clones were used in Southern hybridizations to determine sequence homologies between plasmids of several strains and species.
- Research Organization:
- Miami Univ., Oxford, OH
- OSTI ID:
- 5383688
- Conference Information:
- Journal Name: Plant Physiol.; (United States) Journal Volume: 80:4
- Country of Publication:
- United States
- Language:
- English
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