Interleukin 1 synthesis by a transitional cell carcinoma: relationship to bone resorption and humoral hypercalcemia of malignancy

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5383571

Sammon, P; Wronski, T; Ignaszewski, L; Flueck, J; Cohen, D A

A tumor cell line (TCCB) was isolated from a patient with transitional cell carcinoma, who had tumor-associated hypercalcemia. Culture supernatants from TCCB had significant bone resorption activity in a /sup 45/Ca release assay from fetal mouse long bones. Furthermore, TCCB supernatants possessed potent interleukin 1 (IL-1) activity in a mouse thymocyte assay. Histomorphometric analysis of fetal mouse long bones incubated with TCCB supernatants showed a 7 fold increase in osteoclasts and a significant decrease in bone mass as compared to control bones. IL-1 activity in culture supernatants from lipopolysaccharide-stimulated human monocytes comigrated with both IL-1 and bone resorption activities from TCCB tumor cells by gel chromatography on AcA-54 ultrogel. Furthermore, antisera against human IL-1 was shown to inhibit the Il-1 activity from both monocytes and TCCB tumor cells. In light of recent reports that IL-1 can cause bone resorption in vitro, these data suggest that the hypercalcemia of malignancy seen with certain solid tumors, may be the result of IL-1 release by the tumor which subsequently elevates serum calcium by induction of bone resorption.

• Research Organization: Univ. of Kentucky College of Medicine and Dentistry, Lexington
• OSTI ID: 5383571
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CARCINOMAS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DISEASES
ELEMENTS
EVEN-ODD NUCLEI
FETUSES
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
ISOTOPES
LYMPHOKINES
MAMMALS
METALS
MICE
MITOGENS
NEOPLASMS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PROTEINS
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
SKELETON
SYNTHESIS
TUMOR CELLS
VERTEBRATES