Cadmium-binding protein (metallothionein) in carp

Journal Article · Sat Mar 01 04:00:00 EST 1986 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.2307/3430171· OSTI ID:5381782

Kito, H; Ose, Y; Sato, T

When carp (Cyprinus carpio) were exposed to 5 and 30 ppm Cd in the water, the contents of Cd-binding protein, which has low molecular weight, increased in the hepatopancreas, kidney, gills and gastrointestinal tract with duration of exposure. This Cd-binding protein was purified from hepatopancreas, kidney, gills, and spleen of carp administered 2 mg/kg Cd (as CdCl/sub 2/), intraperitoneally for 6 days. Two Cd-binding proteins were separated by DEAE-Sephadex A-25 column chromatography. These proteins had Cd-mercaptide bond, high cysteine contents (ca. 29-34%), but no aromatic amino acids or histidine. From these characteristics the Cd-binding proteins were identified as metallothionein. By using antiserum obtained from a rabbit to which carp hepatopancreas MT-II had been administered, immunological characteristics between hepatopancreas MT-I, II and kidney MT-II were studied, and a slight difference in antigenic determinant was observed among them. By immunological staining techniques with horseradish peroxidase, the localization of metallothionein was investigated. Carp were bred in 1 ppm Cd, 5 ppm Zn solution, and tap water for 14 days, following transfer to 15 ppm Cd solution, respectively. The survival ratio was the highest in the Zn group followed by Cd-treated and control groups.

• Research Organization: Gifu Pharmaceutical Univ., Japan
• OSTI ID: 5381782
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 65; ISSN EVHPA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CADMIUM CHLORIDES
CADMIUM COMPOUNDS
CADMIUM HALIDES
CHEMISTRY
CHLORIDES
CHLORINE COMPOUNDS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISTRIBUTION
ENDOCRINE GLANDS
FISHES
GASTROINTESTINAL TRACT
GILLS
GLANDS
HALIDES
HALOGEN COMPOUNDS
ION EXCHANGE CHROMATOGRAPHY
KIDNEYS
LIVER
METALLOPROTEINS
METALLOTHIONEIN
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PROTEINS
RESPIRATORY SYSTEM
SEPARATION PROCESSES
SPLEEN
TISSUE DISTRIBUTION
VERTEBRATES