Pheromone induction of agglutination in Saccharomyces cerevisiae a cells

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · J. Bacteriol.; (United States)

OSTI ID:5381526

Terrance, K; Lipke, P N

a-Agglutinin, the cell surface sexual agglutinin of yeast a cells, was assayed by its ability to bind its complementary agglutinin, ..cap alpha..-agglutinin. The specific binding of /sup 125/I-..cap alpha..-agglutinin to a cells treated with the sex pheromone ..cap alpha..-factor was 2 to 2.5 times that of binding to a cells not treated with ..cap alpha..-factor. Competition with unlabeled ..cap alpha..-agglutinin revealed that the increased binding was due to increased cell surface expression of a-agglutinin, with no apparent change in the binding constant. The increase in site number was similar to the increase in cellular agglutinability. Increased expression of a-agglutinin followed the same kinetics as the increase in cellular agglutinability, with a 10-min lag followed by a 15- to 20-min response time. Induction kinetics were similar in cells in phases G1 and G2 of the cell cycle. Maximal expression levels were similar in cells treated with excess pheromone and in cells exposed to pheromone after destruction of constitutively expressed a-agglutinin.

• Research Organization: Hunter College of the City Univ. of New York, NY (USA)
• OSTI ID: 5381526
• Journal Information: J. Bacteriol.; (United States), Vol. 169:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
BIOCHEMICAL REACTION KINETICS
PHEROMONE
CELL CYCLE
IODINE 125
SACCHAROMYCES CEREVISIAE
TRACER TECHNIQUES
ANTIBODIES
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FUNGI
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
PLANTS
RADIOISOTOPES
REACTION KINETICS
SACCHAROMYCES
SECRETION
YEASTS
550201* - Biochemistry- Tracer Techniques