Enhanced unscheduled DNA synthesis in UV-irradiated human skin explants treated with T4N5 liposomes

Journal Article · Mon Jul 01 00:00:00 EDT 1991 · Journal of Investigative Dermatology; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12479314· OSTI ID:5380747

Yarosh, D B; Kibitel, J T; Green, L A; Spinowitz, A ^[1]

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1. Applied Genetics Inc., Freeport, NY (USA)

Epidermal keratinocytes cultured from explants of skin cancer patients, including biopsies from xeroderma pigmentosum patients, were ultraviolet light-irradiated and DNA repair synthesis was measured. Repair capacity was much lower in xeroderma pigmentosum patients than in normal patients. The extent of DNA repair replication did not decline with the age of the normal patient. Treatment with T4N5 liposomes containing a DNA repair enzyme enhanced repair synthesis in both normal and xeroderma pigmentosum keratinocytes in an irradiation- and liposome-dose dependent manner. These results provide no evidence that aging people or skin cancer patients are predisposed to cutaneous malignancy by a DNA repair deficiency, but do demonstrate that T4N5 liposomes enhance DNA repair in the keratinocytes of the susceptible xeroderma pigmentosum and skin cancer population.

• OSTI ID: 5380747
• Journal Information: Journal of Investigative Dermatology; (United States), Vol. 97:1; ISSN 0022-202X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
DNA REPAIR
LIPOSOMES
RADIOSENSITIVITY EFFECTS
SKIN
TRANSPLANTS
BIOLOGICAL RADIATION EFFECTS
MAN
NEOPLASMS
PATIENTS
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
DISEASES
ELECTROMAGNETIC RADIATION
MAMMALS
ORGANS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SKIN DISEASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture