Increased intrapulmonary retention of radiolabeled neutrophils in early oxygen toxicity
Sequential lung injuries, such as oxygen toxicity followed by septicemia, are common during the adult respiratory distress syndrome (ARDS). As these forms of vascular injury may be mediated in part by polymorphonuclear leukocytes (PMN), aberrant interactions between PMN and previously injured pulmonary endothelium are of both theoretical interest and clinical importance. The present study was undertaken to test the hypothesis that early oxygen toxicity at a dose that injuries pulmonary endothelium relatively selectively alters intrapulmonary neutrophil kinetics. Unanesthetized rats breathing 1.0 atmospheres oxygen for 36 h showed ultrastructural endothelial damage but no edema, injury, or neutrophilic inflammation by histologic criteria. However, in these oxygen-toxic animals, whereas initial accumulation of radiolabeled PMN in lungs was normal, washout of PMN was abnormal at 120 min after infusion, at which point the pulmonary retention of radiolabeled PMN in the lungs of oxygen-treated animals was significantly higher than in control animals (139% of control, p less than 0.0096). Features of our methodology, including avoidance of osmotic stress and use of paired control animals, appear to have greatly enhanced the sensitivity of radiolabeled neutrophils for detecting a subtle abnormality of neutrophil-endothelial interactions. Our studies in the oxygen toxicity model provide the first demonstration in vivo of abnormal intrapulmonary neutrophil kinetics in early oxygen toxicity prior to the onset of histologic evidence of lung injury or inflammation.
- Research Organization:
- Univ. of Pittsburgh School of Medicine, PA (USA)
- OSTI ID:
- 5380234
- Journal Information:
- Am. Rev. Respir. Dis.; (United States), Vol. 137:2
- Country of Publication:
- United States
- Language:
- English
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LUNGS
PATHOLOGICAL CHANGES
NEUTROPHILS
RETENTION
OXYGEN
TOXICITY
RESPIRATORY SYSTEM DISEASES
PATHOGENESIS
CELL CYCLE
ENDOTHELIUM
ENDOTOXINS
ESCHERICHIA COLI
ORGANOMETALLIC COMPOUNDS
RADIOISOTOPES
RATS
SEPTICEMIA
TISSUE DISTRIBUTION
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
ANTIGENS
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
DISEASES
DISTRIBUTION
ELEMENTS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
ORGANS
RESPIRATORY SYSTEM
RODENTS
TISSUES
TOXIC MATERIALS
TOXINS
VERTEBRATES
550901* - Pathology- Tracer Techniques