Binding of nuclear factors to the 5 prime -interferon consensus sequence of the HLA-A2 class I gene

Journal Article · Thu Feb 01 04:00:00 EST 1990 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(90)90297-N· OSTI ID:5378471

Le Bouteiller, P; Bogarad, L D; Roberts, M R; Ruddle, F H ^[1]; Barbosa, J A ^[2]

---

1. Yale Univ., New Haven, CT (United States)
2. Molecular Diagnostics, Inc., West Haven, CT (United States)

To investigate the regulatory role of the conserved interferon consensus sequence (ICS) found in the 5{prime} flanking region of HLA class I genes, the authors studied the binding of nuclear proteins to the ICS of HLA-A2 gene (ICS-A2) by the gel shift assay. Nuclear extracts from several human cell lines expressing different levels of surface class I molecules reveal an ICS-A2-protein complex of similar mobility, the amount of which varies in a cell-type=dependent manner. In some cell lines, interferon-{gamma} treatment decreased the level of this complex. The overlapping enhancer A element also competes for the formation of this ICS-A2-protein complex. Footprinting and methylation interference analyses demonstrate that nuclear protein(s) protect specific sequences within the ICS-A2 element, suggesting that these protein(s) may represent interferon-sensitive transcription factors.

• OSTI ID: 5378471
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 186:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
BONE MARROW CELLS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DNA SEQUENCING
FLUORESCENCE
GENE REGULATION
GENE REPRESSORS
GENES
GLYCOPROTEINS
HELA CELLS
HISTOCOMPATIBILITY COMPLEX
LUMINESCENCE
MAMMALS
MAN
METHYLATION
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION FACTORS
TUMOR CELLS
VERTEBRATES