Acetaldehyde increases mRNA levels of glucose-6-phosphate dehydrogenase in rat hepatocytes in culture
- Western Michigan Univ., Kalamazoo (United States) Upjohn Co., Kalamazoo, MI (United States)
Addition of insulin and glucocorticoids for 48h to rat hepatocytes in culture increased glucose-6-phosphate dehydrogenase (G6PDH) activity and mRNA levels. Ethanol was found to potentiate this increase. The addition of ethanol alone for 48h however, had no effect on G6PDH activity or mRNA level. Since the effect of ethanol on G6PDH appears to be dependent on hormones as well as a long incubation time, the data is suggestive of the effect of ethanol being mediated through a metabolite. These studies were therefore initiated to investigate the effects of a major metabolite of ethanol, acetaldehyde. In rat hepatocytes incubated in a chemically defined medium the addition of acetaldehyde increased G6PDH mRNA levels 2-3 fold over control. This increase was observed in as little as 6h of acetaldehyde incubation. When the cells were incubated in the presence of insulin and hydrocortisone for 6h, G6PDH mRNA levels were doubled. Addition of acetaldehyde under these same conditions caused an additional 2-3 fold accumulation of G6PDH mRNA. These effects of acetaldehyde are selective as the control mRNAs were unaffected. Studies are continuing to investigate the mechanism by which acetaldehyde exerts its effects.
- OSTI ID:
- 5374643
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACETALDEHYDE
BIOLOGICAL EFFECTS
ETHANOL
MESSENGER-RNA
BIOSYNTHESIS
OXIDOREDUCTASES
ENZYME ACTIVITY
CELL CULTURES
INSULIN
LIVER CELLS
METABOLISM
ALCOHOLS
ALDEHYDES
ANIMAL CELLS
ENZYMES
HORMONES
HYDROXY COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RNA
SOMATIC CELLS
SYNTHESIS
560300* - Chemicals Metabolism & Toxicology