A colorimetric method for lysyl oxidase activity in copper deficient rats fed a high fructose diet

Conference · Mon Mar 11 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5373087

Werman, M J; Bhathena, S J ^[1]

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1. Dept. of Agriculture, Beltsville, MD (United States)

Lysyl oxidase is involved in initiating cross link formation in collagen and elastin. The activity of lysyl oxidase is traditionally assessed by the tritium released assay. The authors describe a simplified and modified method for measuring lysyl oxidase activity in rats, based on measuring ammonia release according to the Bertholet colorimetric reaction. Lysyl oxidase activity was measured in copper deficient rats using this method. Sixteen weanling Sprague Dawley male rats were fed for four weeks either copper adequate or copper deficient diets containing 62% fructose. Copper deficiency was confirmed by significant low copper levels in heart, brain, liver and skin, and by nondetectable levels of ceruloplasmin. Lysyl oxidase activity was significantly lower in heart and skin of rats fed a copper deficient diet compared to those fed a copper adequate diet. No significant difference in activity was observed in brain tissue. A correlation was not observed between decreased tissue copper levels and decreased lysyl oxidase activity. Thus, the determination of ammonia liberated during lysyl oxidase activity may serve as an effective tool in assessing lysyl oxidase activity.

• OSTI ID: 5373087
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COPPER
BIOLOGICAL EFFECTS
OXIDOREDUCTASES
ENZYME ACTIVITY
DIET
FRUCTOSE
MEASURING METHODS
METABOLISM
NUTRITIONAL DEFICIENCY
RATS
TISSUE DISTRIBUTION
ANIMALS
CARBOHYDRATES
DISTRIBUTION
ELEMENTS
ENZYMES
HEXOSES
KETONES
MAMMALS
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SACCHARIDES
TRANSITION ELEMENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology