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Radioimmunoassay of human high molecular weight kininogen in normal and deficient plasmas

Journal Article · · J. Lab. Clin. Med.; (United States)
OSTI ID:5372082
; ;
An RIA for human HMW kininogen, capable of detecting 150 pg of antigen, has been developed. Antibody to HMW kininogen was purified by immunoaffinity chromatography, and double-antibody precipitation was used to separate free and bound antigen. Of the LMW kininogens only one of the forms tested (B3.2) showed significant cross-reaction (2%). Bradykinin and human plasma kallikrein both showed no cross-reaction, and monkey HMW kininogen showed identity to the human antigen. Intraassay and interassay coefficients of variation were 2 and 1.5%, respectively. Recovery of HMW kininogen added to 6 plasmas was 97.7% +- 1.8%. Assay of 17 normal plasmas gave a level of 90.8 +- 2.5 ..mu..g/ml HMW kininogen (mean +- S.E.M.). A bioassay of the samples, based on specific release of kinin by purified plasma kallikrein, yielded a level of 90.2 +- 2.8 ..mu..g/ml HMW kininogen (r = 0.83, p < 0.001). In neither assay was any significant sex difference observed. No evidence of any antigenic fragments was seen upon gel filtration of normal plasmas. RIA measurements were also performed on seven plasmas reportedly deficient in HMW kininogen. Williams, Dayton, San Francisco, and Flaujeac plasmas all showed no significant cross-reaction, whereas Fitzgerald, Reid, and Detroit plasmas showed 1.0, 2.5, and 3.5% of normal antigenic levels, respectively. This sensitive, convenient method should facilitate studies on the role of the kallikrein-kinin system in health and disease.
Research Organization:
National Heart, Lung, and Blood Inst., Bethesda, MD
OSTI ID:
5372082
Journal Information:
J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 95:4; ISSN JLCMA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
BRADYKININ
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KALLIKREIN
KININS
MAMMALS
MAN
MATERIALS
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
OPTIMIZATION
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PEPTIDES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RESPONSE MODIFYING FACTORS
SPECIFICITY
TRACER TECHNIQUES
VERTEBRATES