Zinc and dexamethasone induce metallothionein accumulation by endothelial cells
- USDA/ARS, Grand Forks, ND (United States)
Several tissues increase their metallothionein (MT) concentration when exposed to elevated amounts of plasma Zn. Endothelial cells form the blood vessels that supply all tissues and constitute a barrier between cells of tissues and the blood. This study examined the ability of endothelial cells to synthesize MT and accumulate Zn in response to high amounts of Zn and dexamethasone. Bovine pulmonary endothelial cells were grown to confluence in Minimum Essential Medium with Earle's salts and 10% fetal calf serum. The monolayer was maintained for 2 d prior to use in medium containing EDTA-dialyzed serum. This low Zn medium was replaced with one containing 1, 6, 25, 50, 100, 150, or 200 {mu}M Zn and incubated for 24 hr before harvesting the cells. MT was quantified by the cadmium binding assay. Cellular Zn concentrations were analyzed by atomic absorption after a nitric acid digestion. The MT concentration was elevated in response to Zn concentrations of 100 {mu}M or more. Cellular Zn concentration was elevated when media Zn was 25 {mu}M or more. MT and cellular Zn concentrations were positively correlated. In another study, inclusion of 0.1 {mu}M dexamethasone in the media increased concentration at all Zn concentrations studied. However, the inclusion of 0.3 {mu}M cis-platinum had no effect. In conclusion, endothelial cells in culture respond to elevated amounts of Zn and dexamethasone in the media by accumulating Zn and MT.
- OSTI ID:
- 5371382
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DEXAMETHASONE
BIOLOGICAL EFFECTS
ENDOTHELIUM
BIOLOGICAL FUNCTIONS
METALLOTHIONEIN
BIOLOGICAL ACCUMULATION
ZINC
ABSORPTION SPECTRA
LUNGS
SHEEP
ADRENAL HORMONES
ANIMAL TISSUES
ANIMALS
BODY
CORTICOSTEROIDS
DOMESTIC ANIMALS
ELEMENTS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
KETONES
MAMMALS
METALLOPROTEINS
METALS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
RESPIRATORY SYSTEM
RUMINANTS
SPECTRA
STEROID HORMONES
STEROIDS
TISSUES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology