Detection of free radical generation in the stunned' myocardium in the conscious dog using spin tripping techniques

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5371185

Zughayb, M; Sekili, S; McCay, P B; Bolli, R ^[1]

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1. Baylor College, Houston, TX (United States) Oklahoma Medical Research Foundation, Oklahoma City (United States)

Recent studies have shown that free radicals (FR) are produced in stunned' myocardium. However, since these studies were performed in open-chest animals, artifacts due to anesthesia, trauma, and other unphysiologic conditions cannot be excluded. FR production in conscious models of myocardial ischemia has never been shown. Thus, conscious dogs undergoing a 15-min coronary occlusion (O) followed by reperfusion (R) received i.v. the spin trap alpha-phenyl N-tert-butyl nitron (PBN) starting 5 min pre-O and ending 10 min after R. Local coronary venous effluent plasma was analyzed by electron paramagnetic resonance (EPR) spectroscopy. Myocardial production of PBN adducts was calculated as coronary flow x venous-arterial difference in EPR signal intensity. A burst of PBN adduct production was observed in the first 5 min of R. Adduct production then abated but remained detectable for several hours after R. Coupling constants are consistent with a complex mixture of FR. In 5 control studies, infusion of PBN without ischemia was not associated with appreciable adduct production. These results demonstrate that reversible regional myocardial ischemia in the conscious animal is associated with free radical generation and further support the hypothesis that oxy-radicals contribute to stunning.

• OSTI ID: 5371185
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

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560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANEMIAS
ANIMALS
BIOLOGICAL STRESS
BIOSYNTHESIS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DISEASES
DOGS
ELECTRON SPIN RESONANCE
HEART
HEMIC DISEASES
ISCHEMIA
MAGNETIC RESONANCE
MAMMALS
MUSCLES
MYOCARDIUM
ORGANS
RADICALS
RESONANCE
SYMPTOMS
SYNTHESIS
VASCULAR DISEASES
VERTEBRATES