Regulation of platelet-activating factor (PAF) receptor and PAF receptor-mediated cellular response in Kupffer cells: Effect of vanadate

Conference · Sun Mar 10 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5370762

Chao, W; Liu, H; Hanahan, D J; Olson, M S ^[1]

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1. Univ. of Texas, San Antonio (United States)

Vanadate is a phosphate analogue which affects phosphate transfer reactions which may be involved in regulatory processes in which tyrosine phosphorylation or dephosphorylation may be an important component. In the present study vanadate decreased the surface expression of PAF receptors and caused tyrosine-phosphorylation in numerous proteins in intact Kupffer cells. The vanadate-induced tyrosine-phosphorylation was inhibited by genistein, a specific tyrosine kinase inhibitor. The EC{sub 50} for the vanadate-initiated decrease in the surface expression of PAF receptors was approximately 0.25 mM, 0.65 mM, and 2 mM, respectively, when the vanadate exposure time was 3 h, 2h, and 1h. As a consequence, PAF-stimulated prostaglandin E{sub 2} (PGE{sub 2}) formation was attenuated in vanadate-treated Kupffer cells. While vanadate itself was found to stimulate PGE{sub 2} production, PAF-stimulated PGE{sub 2}formation was inhibited significantly by genistein. The present study suggests that vanadate stimulated strongly tyrosine-phosphorylation of cellular proteins and decreased the surface expression of PAF receptor in intact Kupffer cells.

• OSTI ID: 5370762
• Report Number(s): CONF-9104107--
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
ELEMENTS
ENZYME INHIBITORS
GLANDS
GROWTH FACTORS
HYDROXY ACIDS
KINETICS
LIVER
MACROPHAGES
METALS
MITOGENS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PHOSPHORYLATION
PROSTAGLANDINS
PROTEINS
REACTION KINETICS
SOMATIC CELLS
SYNTHESIS
TRANSITION ELEMENTS
TYROSINE
VANADIUM