Expression of c-myc gene in human ovary carcinoma cells treated with vanadate
Journal Article
·
· Experimental Cell Research; (United States)
- Engelhardt Inst. of Molecular Biology, Moscow (USSR)
The widely accepted hypothesis of vanadate action on cells postulates that this ion inhibits protein phosphatase(s) that dephosphorylates protein phosphotyrosine residues. This inhibition causes tyrosine hyperphosphorylation of cell proteins followed by changes in physiological action of phosphoproteins resulting in stimulation of cell proliferation, expression of protooncogenes, and transient cell transformation. The authors have found that treatment of human ovary carcinoma (CaOv) cells with vanadate causes the increase in total protein phosphorylation from 1.5- to 2.0-fold whereas the ratio between phosphoserine, phosphothreonine, and phosphotyrosine content remains unchanged. At the same time, enhancement of c-myc gene expression (not c-fos) was observed. Hence, the increase in the ratio of phosphotyrosine to phosphoserine and phosphothreonine is not an obligatory intermediate stage before vanadate-dependent activation of c-myc expression.
- OSTI ID:
- 5312681
- Journal Information:
- Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 188:1; ISSN ECREA; ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
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Sun Mar 10 23:00:00 EST 1991
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
·
OSTI ID:5370762
Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CARCINOMAS
CELL PROLIFERATION
CHEMICAL ACTIVATION
DISEASES
FEMALE GENITALS
FEMALES
GENE REGULATION
GENES
GONADS
HYDROXY ACIDS
MAMMALS
MAN
NEOPLASMS
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OVARIES
OXYGEN COMPOUNDS
PRIMATES
SERINE
THREONINE
TRANSITION ELEMENT COMPOUNDS
TYROSINE
VANADATES
VANADIUM COMPOUNDS
VERTEBRATES
WOMEN
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CARCINOMAS
CELL PROLIFERATION
CHEMICAL ACTIVATION
DISEASES
FEMALE GENITALS
FEMALES
GENE REGULATION
GENES
GONADS
HYDROXY ACIDS
MAMMALS
MAN
NEOPLASMS
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OVARIES
OXYGEN COMPOUNDS
PRIMATES
SERINE
THREONINE
TRANSITION ELEMENT COMPOUNDS
TYROSINE
VANADATES
VANADIUM COMPOUNDS
VERTEBRATES
WOMEN