Is the product of the src gene a promoter

Journal Article · Mon Jan 01 00:00:00 EST 1979 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.76.1.348· OSTI ID:5367571

Bissell, M J; Hatie, C; Calvin, M

Addition of a potent promotor, 12-O-tetradecanoylphorbol 13-acetate (TPA), to primary avian tendon or chicken embryo fibroblast cells infected with a temperature-sensitive mutant of Rous sarcoma virus produced a complete transformed phenocopy at the nonpermissive temperature by the criteria tested. While normal, uninfected cultures also shifted towards a transformed phenotype after TPA addition, they did not achieve the same degree of morphological and biochemical alteration seen in virus-infected, TPA-treated cells. It is proposed that viral carcinogenesis, despite its rapidity, may occur in two stages: an initiation step caused by expression of a part of viral genome other than src (or by integration) and a promotion step (itself a multistep process) caused by the activation of the src gene. The src gene product could be enhanced or replaced by other promoting agents.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 5367571
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 76:1; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ADENOVIRUS
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIRDS
CARBOXYLIC ACID SALTS
CARCINOGENESIS
CELL CULTURES
CHICKENS
CONNECTIVE TISSUE CELLS
DISEASES
FIBROBLASTS
FOWL
GENES
GENOME MUTATIONS
KINETICS
MICROORGANISMS
MORPHOLOGICAL CHANGES
MUTATIONS
NEOPLASMS
ONCOGENIC VIRUSES
PARASITES
PATHOGENESIS
PHENOTYPE
PROMOTERS
REACTION KINETICS
SARCOMAS
SOMATIC CELLS
TEMPERATURE DEPENDENCE
VERTEBRATES
VIRUSES