Cartilage calcification: an ultrastructural, histochemical, and analytical x-ray microprobe study of the zone of calcification in the normal avian epiphyseal growth plate

Journal Article · Mon Mar 01 04:00:00 EST 1982 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/30.3.7061824· OSTI ID:5364444

Davis, W L; Jones, R G; Knight, J P; Hagler, H K

Sections from the zone of calcification of ruthenium red-fixed normal avian epiphyseal growth plates were analyzed by various morphological (histochemical) and analytical techniques. Calcium and phosphorus were identified in the chondrocyte pericellular rim, the uncalcified extracellular (territorial) matrix, and in both the peripheral and central aspects of the calcified accumulations within extracellular matrix. Cartilage proteoglycan, as determined by ruthenium binding, positive staining with acidic phosphotungstic acid, and the X-ray spectroscopic detection of sulfur, was identified in the same four zones. Thus, it appears that proteoglycans, in some form, are indeed retained at sites of biological calcification. Additionally, these macromolecules, synthesized in chondrocytes, may be involved in extracellular calcium translocation.

• Research Organization: Department of Microscopic Anatomy, Baylor College of Dentistry, University of Texas Health Science Center, Southwestern Medical School, Dallas
• OSTI ID: 5364444
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 30:3; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550301 -- Cytology-- Tracer Techniques
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMAL TISSUES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
BONE TISSUES
CALCIUM
CONNECTIVE TISSUE
DYNAMIC FUNCTION STUDIES
ELEMENTS
KINETICS
METALS
REACTION KINETICS
SYNTHESIS
TISSUES