Excision repair of UV radiation-induced DNA damage in Caenorhabditis elegans
Journal Article
·
· Genetics; (USA)
OSTI ID:5363931
- Texas Christian Univ., Fort Worth (USA)
Radioimmunoassays were used to monitor the removal of antibody-binding sites associated with the two major UV radiation-induced DNA photoproducts (cyclobutane dimers and (6-4) photoproducts). Unlike with cultured human cells, where (6-4) photoproducts are removed more rapidly than cyclobutane dimers, the kinetics of repair were similar for both lesions. Repair capacity in wild type diminished throughout development. The radioimmunoassays were also employed to confirm the absence of photoreactivation in C. elegans. In addition, three radiation-sensitive mutants (rad-1, rad-2, rad-7) displayed normal repair capacities. An excision defect was much more pronounced in larvae than embryos in the fourth mutant tested (rad-3). This correlates with the hypersensitivity pattern of this mutant and suggests that DNA repair may be developmentally regulated in C. elegans. The mechanism of DNA repair in C. elegans as well as the relationship between the repair of specific photoproducts and UV radiation sensitivity during development are discussed.
- OSTI ID:
- 5363931
- Journal Information:
- Genetics; (USA), Journal Name: Genetics; (USA) Vol. 122:2; ISSN 0016-6731; ISSN GENTA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ASCHELMINTHES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMISTRY
DIAGNOSTIC TECHNIQUES
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
EXCISION REPAIR
HELMINTHS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KINETICS
LARVAE
MEMBRANE PROTEINS
MUTANTS
NEMATODES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOTOCHEMISTRY
PROTEINS
PYRIMIDINE DIMERS
RADIATION INDUCED MUTANTS
RADIATIONS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
REACTION KINETICS
RECEPTORS
RECOVERY
REPAIR
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
59 BASIC BIOLOGICAL SCIENCES
ASCHELMINTHES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMISTRY
DIAGNOSTIC TECHNIQUES
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
EXCISION REPAIR
HELMINTHS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KINETICS
LARVAE
MEMBRANE PROTEINS
MUTANTS
NEMATODES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOTOCHEMISTRY
PROTEINS
PYRIMIDINE DIMERS
RADIATION INDUCED MUTANTS
RADIATIONS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
REACTION KINETICS
RECEPTORS
RECOVERY
REPAIR
TRACER TECHNIQUES
ULTRAVIOLET RADIATION