Quantitation of postischemic skeletal muscle necrosis: histochemical and radioisotope techniques

Journal Article · Fri Jan 01 00:00:00 EST 1988 · J. Surg. Res.; (United States)

DOI:https://doi.org/10.1016/0022-4804(88)90121-7· OSTI ID:5362358

Labbe, R; Lindsay, T; Gatley, R; Romaschin, A; Mickle, D; Wilson, G; Houle, S; Walker, P

Skeletal muscle necrosis will result from prolonged periods of ischemia. The purpose of this study was to develop a method to estimate the extent of necrosis using nitroblue tetrazolium staining and technetium scanning. The bilateral canine gracilis muscle preparation with total vascular isolation was exposed to 4 hr of complete normothermic ischemia followed by reperfusion. After 45 hr of reperfusion /sup 99m/Tc pyrophosphate (PYP) was injected and 3 hr later the muscles were harvested, cut into six slices, and stained with nitroblue tetrazolium. Biopsies were taken from tetrazolium-positive and -negative areas for electron microscopy to confirm the ability of the stain to distinguish viable from necrotic muscle. Computerized planimetry of the staining pattern was used to estimate the extent of necrosis as a percentage of the total muscle. Electron microscopy confirmed the validity of nitroblue tetrazolium to discriminate between viable and necrotic skeletal muscle in this experimental model. After 4 hr of ischemia the percentage necrosis was 30.2 +/- 6.1% (mean +/- SEM, n = 12), there was no difference in the extent of necrosis in left vs right paired muscles, using tetrazolium staining or technetium PYP uptake. There was a statistically significant correlation between the percentage necrosis and the density of 99mTc PYP uptake per muscle (r = 0.83, P less than 0.001) and per slice (r = 0.94, P less than 0.001). This study demonstrates the ability of tetrazolium staining to accurately differentiate between viable and necrotic skeletal muscle and provides a reproducible method for estimating the extent of necrosis in the gracilis muscle model.

• Research Organization: Toronto General Hospital, Ontario
• OSTI ID: 5362358
• Journal Information: J. Surg. Res.; (United States), Vol. 44:1
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ISCHEMIA
PATHOGENESIS
MUSCLES
NECROSIS
NUCLEAR MEDICINE
TRACER TECHNIQUES
SKELETAL DISEASES
DIAGNOSIS
BIOLOGICAL MODELS
DOGS
ELECTRON MICROSCOPY
IN VITRO
ISOMERIC NUCLEI
MEASURING METHODS
PYROPHOSPHATES
RADIOISOTOPE SCANNING
STAINS
TECHNETIUM 99
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARDIOVASCULAR DISEASES
COUNTING TECHNIQUES
DISEASES
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEDICINE
MICROSCOPY
NUCLEI
ODD-EVEN NUCLEI
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PHOSPHORUS COMPOUNDS
RADIOISOTOPES
TECHNETIUM ISOTOPES
VASCULAR DISEASES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics