Sustained benefit of temporary limited reperfusion in skeletal muscle following ischemia
- Univ. of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark (USA)
Limiting the rate of reperfusion blood flow following prolonged ischemia in skeletal muscle has been shown beneficial. However, the persistence of this benefit with reinstitution of normal blood flow remains undefined. We investigated the role of temporary limited reperfusion on ischemia-reperfusion injury in an isolated gracilis muscle model in six anesthetized dogs. Both gracilis muscles were subjected to 6 hr of ischemia followed by 2 hr of reperfusion. Reperfusion blood flow was limited for the first hour in one gracilis muscle to its preischemic rate followed by a second hour of normal reperfusion (LR/NR). The contralateral muscle underwent 2 hr of normal reperfusion (NR/NR). Muscle injury was quantified by technetium-99m pyrophosphate (TcPyp) uptake and by histochemical staining using triphenyltetrazolium chloride (TTC) with planimetry of the infarct size. Capillary permeability was evaluated by muscle weight gain. Results are reported as the mean +/- SEM. These data demonstrate a sustained benefit from temporary limited reperfusion. This methodology should be considered in the surgical management of the acutely ischemic limb.
- OSTI ID:
- 6509608
- Journal Information:
- Journal of Surgical Research; (USA), Vol. 49:3; ISSN 0022-4804
- Country of Publication:
- United States
- Language:
- English
Similar Records
Technetium 99m pyrophosphate quantitation of skeletal muscle ischemia and reperfusion injury
Quantification of myocardial injury produced by temporary coronary artery occlusion and reflow with technetium-99m-pyrophosphate
Related Subjects
CAPILLARIES
PERMEABILITY
ISCHEMIA
PATHOGENESIS
MUSCLES
BLOOD FLOW
BLOOD PRESSURE
DOGS
ISOMERIC NUCLEI
PERFUSED TISSUES
PYROPHOSPHATES
TECHNETIUM 99
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DISEASES
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
RADIOISOTOPES
TECHNETIUM ISOTOPES
TISSUES
VASCULAR DISEASES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550901* - Pathology- Tracer Techniques