Fermentation to ethanol of xylose present in biomass byproduct solutions. Progress report, first year, April 1-December 31, 1981

Technical Report · Wed Dec 31 23:00:00 EST 1980

OSTI ID:5359051

Highlights of progress during the period are summarized under the following headings: characterization of xylose transport in Schizosaccharomyces pombe; growth of s. pombe on xylulose; cloning of xylose isomerase gene from Rhotorula glutenis and Escherichia coli; Radioassay of xylulokinase; and growth of s. pombe in spent sulfite liquor. The objective of the program is to construct a yeast strain capable of fermenting xylose using genetic engineering methods and to maximize ethanol production from xylose in spent sulfite liquor from paper mills and in hemicellulose acid hydrolysates from agricultural wastes. (DMC)

• Research Organization: Washington Univ., Seattle (USA). Dept. of Genetics
• DOE Contract Number: AT06-81ER10879
• OSTI ID: 5359051
• Report Number(s): DOE/ER/10879-1; ON: DE82013703
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALDEHYDES
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
CARBOHYDRATES
CELL CONSTITUENTS
CLONING
CONTROL
DNA
DNA-CLONING
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
ETHANOL
FERMENTATION
FUNGI
GENETIC CONTROL
HYDROXY COMPOUNDS
INDUSTRIAL WASTES
ISOMERASES
LIQUID WASTES
MEMBRANE TRANSPORT
MICROORGANISMS
MONOSACCHARIDES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PENTOSES
PEST CONTROL
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PLANTS
PLASMIDS
RADIOASSAY
RECOMBINANT DNA
SACCHARIDES
SPENT LIQUORS
SYNTHESIS
TRANSFERASES
WASTES
XYLOSE
YEASTS