Repair of radiation-induced DNA damage in rat epidermis as a function of age

Journal Article · Wed May 01 04:00:00 EDT 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576464· OSTI ID:5355795

Sargent, E V; Burns, F J

The rate of repair of radiation-induced DNA damage in proliferating rat epidermal cells diminished progressively with increasing age of the animal. The dorsal skin was irradiated with 1200 rad of 0.8 MeV electrons at various ages, and the amount of DNA damage was determined as a function of time after irradiation by the method of alkaline unwinding followed by S/sub 1/ nuclease digestion. The amount of DNA damage immediately after irradiation was not age dependent, while the rate of damage removal from the DNA decreased with increasing age. By fitting an exponential function to the relative amount of undamaged DNA as a function of time after irradiation, DNA repair halftimes of 20, 27, 69, and 107 min were obtained for 28, 100-, 200-, and 400-day-old animals, respectively.

• Research Organization: New York Univ. Medical Center, NY
• DOE Contract Number: AC02-76EV03380
• OSTI ID: 5355795
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 102:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560114* -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
DNA
DNA REPAIR
DNA-ASE
ELECTRONS
ELEMENTARY PARTICLES
ENERGY RANGE
ENZYMES
EPIDERMIS
EPITHELIUM
ESTERASES
FERMIONS
HYDROLASES
LABELLED COMPOUNDS
LEPTONS
MAMMALS
MEV RANGE
MEV RANGE 01-10
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHODIESTERASES
RADIATION EFFECTS
RATS
RECOVERY
REPAIR
RODENTS
SKIN
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES