DNA double strand breaks in rat epidermis following irradiation with electrons
Technical Report
·
OSTI ID:5617546
Although radiation induced single strand breaks in rat epidermis are repaired fairly quickly (t-1/2 = 21 minutes), the fate of DNA double strand breaks in the same cells is unclear. Here we have attempted to measure dsb's in rat epidermis by neutral elution. The DNA of 28 day old CD rats was prelabeled with 6 I.P. injections at 2.0 uCi/g body weight of /sup 3/H-TdR. The dorsal skin was irradiated with a 0.8 MeV electron beam. The epidermis was removed by trypsinization at 4/sup 0/C and a single cell suspension was made. The cells were layered onto a polycarbonate filter, lysed, and eluted at pH 9.6. Doses of at least 6000 rads were needed to detect dsb's in vivo. Dsb's were still detectable in the epidermis 3 hours after irradiation. The amount of dsb's had returned to non-irradiated levels 8 hours after irradiation. 77 refs., 2 figs., 1 tab.
- Research Organization:
- New York Univ., NY (USA). Dept. of Environmental Medicine
- DOE Contract Number:
- AC02-76EV03380
- OSTI ID:
- 5617546
- Report Number(s):
- DOE/EV/03380-47; ON: DE86012684
- Country of Publication:
- United States
- Language:
- English
Similar Records
The effect of radiation penetration on DNA single-strand breaks in rat skin explants
DNA single-strand breaks, double-strand breaks, and crosslinks in rat testicular germ cells: Measurements of their formation and repair by alkaline and neutral filter elution
Measurements by filter elution of DNA single- and double-strand breaks in rat hepatocytes: effects of nitrosamines and gamma-irradiation
Technical Report
·
Sat Dec 31 23:00:00 EST 1988
·
OSTI ID:5561134
DNA single-strand breaks, double-strand breaks, and crosslinks in rat testicular germ cells: Measurements of their formation and repair by alkaline and neutral filter elution
Journal Article
·
Sat Jun 01 00:00:00 EDT 1985
· Cell Biology and Toxicology; (USA)
·
OSTI ID:6983397
Measurements by filter elution of DNA single- and double-strand breaks in rat hepatocytes: effects of nitrosamines and gamma-irradiation
Journal Article
·
Thu Jul 01 00:00:00 EDT 1982
· Cancer Res.; (United States)
·
OSTI ID:7067529
Related Subjects
560154* -- Radiation Effects on Animals-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
DATA
ELECTRONS
ELEMENTARY PARTICLES
EXPERIMENTAL DATA
FERMIONS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEPTONS
MAMMALS
NUMERICAL DATA
ORGANS
RADIATION EFFECTS
RATS
RECOVERY
REPAIR
RODENTS
SKIN
STRAND BREAKS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
DATA
ELECTRONS
ELEMENTARY PARTICLES
EXPERIMENTAL DATA
FERMIONS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEPTONS
MAMMALS
NUMERICAL DATA
ORGANS
RADIATION EFFECTS
RATS
RECOVERY
REPAIR
RODENTS
SKIN
STRAND BREAKS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES