Promotion of septation in irradiated Escherichia coli by a cytoplasmic membrane preparation

Journal Article · Wed May 01 04:00:00 EDT 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576470· OSTI ID:5355555

Gill, J S; Adler, H I

Septation can be promoted in an X-irradiated lon mutant of Escherichia coli K-12 by the addition of an E. coli B/r cytoplasmic membrane preparation to the postirradiation plating medium. The promotion of septation was not associated with an inhibition of growth rate. Two distinct cytoplasmic membrane-associated properties were necessary to promote septation. One of these, the cytochrome-based electron transport system, produced anaerobic conditions by the reduction of oxygen dissolved in the medium. The second system, functioning independently from the first, altered substances found in the peptone and yeast extract components of the postirradiation plating medium. When both systems were operative, significant repair of the cell division mechanism occurred.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5355555
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 102:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CELL DIVISION
CELL MEMBRANES
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
IONIZING RADIATIONS
MEMBRANES
MICROORGANISMS
MUTANTS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
X RADIATION