Kinetic study on enzymatic hydrolysis of cellulose by cellulase from Trichoderma viride

Journal Article · Mon Aug 01 04:00:00 EDT 1983 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260250813· OSTI ID:5349909

Ohmine, K; Ooshima, H; Harano, Y

Pure cellulose (Avicel) was hydrolyzed batchwise at 50 degrees C and pH 4.8 by cellulase from Trichoderma viride (Meicelase CEP). Then the effects of the crystallinity of cellulose as well as the thermal deactivation and product (cellobiose and glucose) inhibition to cellulase on the hydrolysis rate were quantitatively investigated. While these factors had evidently retarded the enzymatic hydrolysis of cellulose to a significant extent, the hydrolysis rates observed could not be explained. For practical purposes, an empirical, simple rate expression was developed which included only one parameter: an overall rate retardation constant. This empirical rate expression held for the hydrolysis of at least two kinds of cellulosic materials: Avicel and tissue paper. (Refs. 10)

• Research Organization: Osaka City Univ., Japan
• OSTI ID: 5349909
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 25:8; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
CARBOHYDRATES
CELLOBIOSE
CELLULASE
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
ENZYMATIC HYDROLYSIS
ENZYMES
FUNGI
GLUCOSE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
INHIBITION
KINETICS
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
TRICHODERMA
TRICHODERMA VIRIDE