Isolation and characterization of mammalian glycosylation mutants

Thesis/Dissertation · Wed Jan 01 04:00:00 EST 1986

OSTI ID:5349201

Stoll, J

Isolation and characterization of Chinese Hamster Ovary mutants was done to better understand the pathway of Asn-linked glycosylation. B4-2-1 cells were previously isolated as being defective in the membrane glycoprotein, the mannose-6-phosphate receptor. These cells are shown to be defective in the synthesis of mannosylphosphoryldolichol (MPD) and also mannosylphosphorylretinol. The absence of MPD results in the synthesis of a single major oligosaccharide containing only five mannoses. This oligosaccharide is not a substrate for endo-beta-N-acetylglucosaminidase F, although when present on the protein the oligosaccharide is capable of being released by peptide-N-glycosidase F. B4-2-1 was the parent cell for three new mutant cell lines. These cell lines were isolated by a screening procedure which monitored the incorporation of (/sup 3/H) mannose into acid-precipitable material. Two classes of mutant were obtained as defined by in vivo incorporation of (/sup 3/H) mannose.

• Research Organization: Johns Hopkins Univ., Baltimore, MD (USA)
• OSTI ID: 5349201
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOHYDRATES
CHO CELLS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MANNOSE
METABOLISM
MONOSACCHARIDES
MUTANTS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS