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Effects of preventing O-glycosylation on the secretion of human chorionic gonadotropin in Chinese hamster ovary cells

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Human chorionic gonadotropin (hCG) is a member of a family of heterodimeric glycoprotein hormones that have a common ..cap alpha.. subunit but differ in their hormone-specific ..beta..-subunits. The ..beta.. subunit of hCG (hCG..beta..) is unique among the ..beta.. subunits in that it contains four mucin-like O-linked oligosaccharides attached to a carboxyl-terminal extension. To study the effects of O-glycosylation on the secretion and assembly of hCG, expression vectors containing either hCG..beta.. gene alone or together with the hCG..cap alpha.. gene were transfected into a mutant Chinese hamster ovary cell line, 1d1D, which exhibits a reversible defect in O-glycosylation. The results reveal that hCG..beta.. can be secreted normally in the absence of its O-linked oligosaccharides. hCG..beta.. devoid of O-linked carbohydrate can also combine efficiently with hCG..cap alpha.. and be secreted as an intact dimer. The authors conclude that in Chinese hamster ovary cells, the hCG..beta.. O-linked chains play no role in the assembly and secretion of hCG. The normal and O-linked oligosaccharide-deficient forms of hCG secreted by these cells should prove useful in examining the role of O-linked chains on the biological function of hCG.

Research Organization:
Washington Univ. School of Medicine, St. Louis, MO (USA)
OSTI ID:
7143415
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:18; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHO CELLS
CYSTEINE
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
ENZYMES
EVEN-ODD NUCLEI
GENE RECOMBINATION
GENES
GLUCOPROTEINS
GLYCOSYL HYDROLASES
GONADOTROPINS
HCG
HORMONES
HYDROLASES
HYDROLYSIS
IMMUNE REACTIONS
ISOTOPES
LIGHT NUCLEI
LYSIS
NUCLEI
NUCLEIC ACIDS
O-GLYCOSYL HYDROLASES
OLIGONUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
POST-TRANSLATION MODIFICATION
PROTEINS
RADIOISOTOPES
SACCHARIDES
SECRETION
SOLVOLYSIS
SULFUR 35
SULFUR ISOTOPES
THIOLS