Method for sequencing DNA base pairs

Patent · Tue Dec 14 00:00:00 EST 1993

OSTI ID:5349198

Sessler, A M; Dawson, J

The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source. 6 figures.

• DOE Contract Number: AC03-76SF00098
• Assignee: Dept. of Energy, Washington, DC (United States)
• Patent Number(s): US 5270214; A
• OSTI ID: 5349198
• Resource Relation: Patent File Date: 30 May 1990
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA SEQUENCERS
DNA SEQUENCING
SCANNING ELECTRON MICROSCOPY
INFRARED RADIATION
MOLECULAR STRUCTURE
OLIGONUCLEOTIDES
THREE-DIMENSIONAL CALCULATIONS
ELECTROMAGNETIC RADIATION
ELECTRON MICROSCOPY
EQUIPMENT
LABORATORY EQUIPMENT
MICROSCOPY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry
550400 - Genetics