Method for sequencing DNA base pairs

Patent · 31 December 1992

OSTI ID:869068

Sessler, Andrew M ^[1]; Dawson, John ^[2]

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1. Oakland, CA
2. Pacific Palisades, CA

The base pairs of a DNA structure are sequenced with the use of a scanning tunneling microscope (STM). The DNA structure is scanned by the STM probe tip, and, as it is being scanned, the DNA structure is separately subjected to a sequence of infrared radiation from four different sources, each source being selected to preferentially excite one of the four different bases in the DNA structure. Each particular base being scanned is subjected to such sequence of infrared radiation from the four different sources as that particular base is being scanned. The DNA structure as a whole is separately imaged for each subjection thereof to radiation from one only of each source.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA
• DOE Contract Number: AC03-76SF00098
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 5270214
• OSTI ID: 869068
• Country of Publication: United States
• Language: English

Related Subjects

/436/250/
base
base pair
base pairs
bases
dna
dna structure
excite
imaged
infrared
infrared radiation
method
microscope
pairs
particular
preferentially
preferentially excite
probe
probe tip
radiation
scanned
scanning
scanning tunneling
selected
separately
sequence
sequenced
sequencing
sequencing dna
source
sources
stm
structure
subjected
subjection
tip
tunneling
tunneling microscope