A biochemical defect in the repair of alkylated DNA in cells from an immunodeficient patient (46BR)
Journal Article
·
· Carcinogenesis (N.Y.); (United States)
The fibroblast cell strain 46BR, derived from an immunodeficient individual, is hypersensitive to the lethal effects of a variety of DNA-damaging agents, this effect being particularly marked for monofunctional methylating agents. After U.V. irradiation 46BR cells show normal unscheduled DNA synthesis, daughter strand repair, and recovery of DNA and RNA synthesis. The inhibition of DNA replicative synthesis by U.V. is slightly less than that of normal cells. After gamma-irradiation the rejoining of strand breaks is normal as are the kinetics of replicative DNA synthesis. Following treatment with dimethylsulphate, replicative DNA synthesis is affected in a similar way to normal cells, unscheduled DNA synthesis may be increased relative to normal cells, but more strand breaks persist in 46BR than in normal cells. In addition 46BR cells are hypersensitive to the toxic effects of 3-aminobenzamide, an inhibitor of ADP-ribosyl transferase. This enzyme is involved in the ligation step of repair of alkylation damage. A hypothesis is presented suggesting that 46BR may be defective in DNA ligase I.
- Research Organization:
- MRC Cell Mutation Unit, Sussex University, Brighton, UK
- OSTI ID:
- 5348752
- Journal Information:
- Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 4:5; ISSN CRNGD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Differences in the regulation by poly(ADP-ribose) of repair of DNA damage from alkylating agents and ultraviolet light according to cell type
Cells from an immunodeficient patient (46BR) with a defect in DNA ligation are hypomutable but hypersensitive to the induction of sister chromatid exchanges
Effect of 3-aminobenzamide on the rate of ligation during repair of alkylated DNA in human fibroblasts
Journal Article
·
Wed Aug 10 00:00:00 EDT 1983
· J. Biol. Chem.; (United States)
·
OSTI ID:6241557
Cells from an immunodeficient patient (46BR) with a defect in DNA ligation are hypomutable but hypersensitive to the induction of sister chromatid exchanges
Journal Article
·
Sun Mar 31 23:00:00 EST 1985
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6377676
Effect of 3-aminobenzamide on the rate of ligation during repair of alkylated DNA in human fibroblasts
Journal Article
·
Fri Jul 01 00:00:00 EDT 1983
· Cancer Res.; (United States)
·
OSTI ID:5348890
Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMINES
ANIMAL CELLS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CONNECTIVE TISSUE CELLS
CYTOSINE
DNA
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
FIBROBLASTS
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
IMMUNITY
INJURIES
IONIZING RADIATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATION INJURIES
RADIATIONS
RECOVERY
REPAIR
SENSITIVITY
SOMATIC CELLS
STRAND BREAKS
ULTRAVIOLET RADIATION
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMINES
ANIMAL CELLS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CONNECTIVE TISSUE CELLS
CYTOSINE
DNA
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
FIBROBLASTS
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
IMMUNITY
INJURIES
IONIZING RADIATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATION INJURIES
RADIATIONS
RECOVERY
REPAIR
SENSITIVITY
SOMATIC CELLS
STRAND BREAKS
ULTRAVIOLET RADIATION