Phosphorylation as a method of regulating the activity of E. coli inorganic pyrophosphatase. I. Phosphorylation and activation of the enzyme under the action of ATP

Journal Article · Mon Dec 01 00:00:00 EST 1986 · Sov. J. Bioorg. Chem. (Engl. Transl.); (United States)

OSTI ID:5347614

Vener, A V; Ichetovkina, L E; Nazarova, T I; Avaeva, S M

ATP phosphorylates the regulatory site of E. coli inorganic pyrophosphatase, which leads to a 1.5-fold increase in the activity of the enzyme. The maximum inclusion of ..gamma..-phosphate groups from ATP into the pyrophosphatase is 3 mole per mole of protein. Orthophosphate also phosphorylates the regulatory site of the enzyme, causing a 10-15% fall in the activity of the pyrophosphatase. Phosphorylation under the action of ATP and of orthophosphate are interdependent: ATP prevents the phosphorylation of E. coli inorganic pyrophosphatase under the action of orthosphosphate and leads to a rapid dephosphorylation of protein previously modified with orthosphosphate.

• Research Organization: M. V. Lomonosov Moscow State Univ. (USSR)
• OSTI ID: 5347614
• Journal Information: Sov. J. Bioorg. Chem. (Engl. Transl.); (United States), Vol. 12:2; Other Information: Translated from Bioorganicheskaya Khim.; 12: No. 2, 195-199(Feb 1986)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ATP
ACTIVATION ENERGY
PHOSPHATASES
ENZYME ACTIVITY
METABOLIC ACTIVATION
PHOSPHORYLATION
RADIOENZYMATIC ASSAY
BINDING ENERGY
BIOCHEMISTRY
CARBON 14 COMPOUNDS
CATIONS
ENZYMATIC HYDROLYSIS
ESCHERICHIA COLI
LABELLING
PHOSPHORIC ACID
PHOSPHORUS 32
PROTEINS
PYROPHOSPHATES
SCINTILLATION COUNTING
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHARGED PARTICLES
CHEMICAL REACTIONS
CHEMISTRY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ENERGY
ENZYMES
ESTERASES
HYDROGEN COMPOUNDS
HYDROLASES
HYDROLYSIS
INORGANIC ACIDS
IONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LYSIS
MICROORGANISMS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SOLVOLYSIS
550201* - Biochemistry- Tracer Techniques