Apparent decrease in thioTEPA-induced chromosome aberrations in human lymphocytes caused by an effect of WR2721 on the cell cycle as found by the definitively determined division method

Journal Article · Mon Jan 01 00:00:00 EST 1979 · Environmental Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2860010106· OSTI ID:5338487

Wolff, S; Arutyunyan, R

Antimutagenic radioprotective compounds have been reported to decrease the yield of chemically induced chromosome aberrations even when administered long before the chemical mutagen thio-TEPA. Because thio-TEPA can induce aberrations in all parts of the cell cycle, it seemed likely that the apparent decrease in aberrations was the result of an effect of the antimutagen on the progression of cells through the cell cycle so that cells treated in the more sensitive stage would be scored. To test this possibility human lymphocytes were treated with the protective compound WR2721 and then thio-TEPA. The cells were grown in the presence of 5-bromodeoxyuridine, which allows the definitive determination of metaphases from cells that divided once, twice, or three times after treatment. The yield of aberrations observed in first division cells was the same whether or not the protector was present. A decrease in aberration yields appeared only in rapidly cyling cells that were in the second division at the time of fixation. Labeling experiments showed that in this rapidly dividing population fewer of the metaphase cells had been in G/sub 2/ when thio-TEPA was added. The results indicate that part of the decrease in aberration yields obtained by treatment with an antimutagen several hours before the addition of a mutagen is an artifact of cell selection.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 5338487
• Journal Information: Environmental Mutagenesis; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LYMPHOCYTES
CHROMOSOMAL ABERRATIONS
RADIOPROTECTIVE SUBSTANCES
BIOLOGICAL EFFECTS
ANTINEOPLASTIC DRUGS
CELL CULTURES
CELL CYCLE
MAN
MUTAGENESIS
MUTAGENS
MUTATION FREQUENCY
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DRUGS
LEUKOCYTES
MAMMALS
MATERIALS
MUTATIONS
PRIMATES
RESPONSE MODIFYING FACTORS
SOMATIC CELLS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
560121 - Radiation Effects on Cells- External Source- (-1987)