A microsuspension adaptation of the Bacillus subtilis ''rec'' assay. [Detection of chemically induced DNA damage]

Journal Article · Thu Jan 01 00:00:00 EST 1981 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860030603· OSTI ID:5336540

McCarroll, N E; Keech, B H; Piper, C E

We have demonstrated the utility of an Escherichia coli microsuspension assay to detect and characterize chemical mediation of DNA damage by a wide variety of mutagens and carcinogens. The assay have been improved by the development of a microsuspension modification to the Bacillus subtilis ''rec'' assay. The addition of these gram-positive organisms has allowed detection of DNA damage induced by benzo(a)pyrene (B(a)P), 3-aminopyrene (3-AP), 7, 12-dimethylbenz(a)anthrancene (DMBA), 3-methylcholanthrene (3-MC), and 4-nitrobiphenyl (4-NBP). Data presented in this paper from tests of 61 additional compounds, including a representative number of direct and promutagenic agents, indicate that the B subtilis H17 and M45 strains provide an effective microbial system for identification of DNA damage susceptible to postreplicational repair. The results of this study further suggests that the inclusion of these strains in the microsuspension assay for DNA damage will markedly enhance the detection of agents which cannot readily penetrate the intact cell wall of E coli.

• Research Organization: Hazleton Laboratories America, Inc., Vienna, VA
• OSTI ID: 5336540
• Journal Information: Environ. Mutagen.; (United States), Vol. 3:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARCINOGEN SCREENING
EVALUATION
DNA
INJURIES
MUTAGEN SCREENING
3-METHYLCHOLANTHRENE
BACILLUS SUBTILIS
BENZANTHRACENE
BENZOPYRENE
BIOASSAY
BIPHENYL
ESCHERICHIA COLI
HOMOGENATES
LIVER
METABOLIC ACTIVATION
NITRO COMPOUNDS
RATS
ANIMALS
AROMATICS
BACILLUS
BACTERIA
BODY
CONDENSED AROMATICS
DIGESTIVE SYSTEM
GLANDS
HYDROCARBONS
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
POLYCYCLIC AROMATIC HYDROCARBONS
RODENTS
SCREENING
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)