Capacity for postreplication repair correlated with transducibility in Rec/sup -/ mutants of Bacillus subtilis

Journal Article · Sat Nov 01 04:00:00 EST 1980 · J. Bacteriol.; (United States)

OSTI ID:7097373

Dodson, L A; Hadden, C T

Bacillus subtilis strains deficient in transduction, transformation, or both were examined for the ability to remove pyrimidine dimers and to convert deoxyribonucleic acid newly synthesized after ultraviolet irradiation to high molecular weight. In one strain deficient in both recombination processes, short pieces of deoxyribonucleic acid synthesized after irradiation were not converted to high molecular weight. Two transformable strains deficient in transduction were also deficient in postreplication repair (i.e., joining of newly synthesized DNA fragments), whereas a nontransformable strain that was normal in transduction was proficient in postreplication repair. None of the transformable strains showed deficiencies in repair resynthesis or ligase activity. Our results suggest that some recombinational events may be common to transduction and postreplication repair but not to transformation, emphasizing the difference between these two pathways for genetic exchange.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 7097373
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 144:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560130* -- Radiation Effects on Microorganisms
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BACILLUS
BACILLUS SUBTILIS
BACTERIA
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CHROMOSOMAL ABERRATIONS
DIMERS
DNA
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
GENE RECOMBINATION
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
LIGASES
MICROORGANISMS
MOLECULAR WEIGHT
MUTANTS
MUTATIONS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SENSITIVITY
ULTRAVIOLET RADIATION