Testosterone metabolism of fibroblasts grown from prostatic carcinoma, benign prostatic hyperplasia and skin fibroblasts
The metabolism of (1,2,6,7-3H)testosterone was assessed in fibroblast monolayers derived from tissue of 5 prostates with benign hyperplasia (BPH), 4 prostates with carcinoma (PC), and 3 biopsy samples of skin, 2 nongenital skin (NG) and 1 genital skin. The following metabolites could be identified: androstanedione androstenedione, dihydrotestosterone, androsterone, epiandrosterone, androstane-3 alpha, 17 beta-diol and androstane-3 beta, 17 beta-diol. Testosterone was metabolized much more rapidly in fibroblasts originating from prostatic tissue than in fibroblasts derived from NG. A significantly higher formation of 5 alpha-androstanes and 3 alpha-hydroxysteroids could be observed in fibroblasts from BPH as compared to PC. 17-ketosteroid formation exceeded 5 alpha-androstane formation in BPH, whereas 5 alpha-reduction was the predominant pathway in fibroblasts grown from PC and NG. Since testosterone metabolism in fibroblasts of prostatic origin therefore resembles in many aspects that in whole prostatic tissue, fibroblasts grown from prostatic tissues might be a valuable tool for further investigation of the pathogenesis of human BPH and PC.
- Research Organization:
- Department of Internal Medicine, Medizinische Universitaets-Poliklinik, University of Bonn, West Germany
- OSTI ID:
- 5335622
- Journal Information:
- J. Urol.; (United States), Journal Name: J. Urol.; (United States) Vol. 127:2; ISSN JOURA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMALS
BODY
CARCINOGENESIS
CARCINOMAS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DISEASES
FIBROBLASTS
GLANDS
HORMONES
HYDROXY COMPOUNDS
IN VITRO
KETONES
LABELLED COMPOUNDS
MALE GENITALS
MAMMALS
MAN
METABOLISM
METABOLITES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PRIMATES
PROSTATE
SEPARATION PROCESSES
SKIN
SOMATIC CELLS
STEROID HORMONES
STEROIDS
TESTOSTERONE
THIN-LAYER CHROMATOGRAPHY
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES