Factors influencing the expression of T15 idiotopes during an antigen-driven response to phosphorylcholine (PC)
The idiotypy of the serum antibody response to PC was examined in twelve inbred strains of mice including several Igh- and h-2 congenics. The sera were collected after primary (1/sup 0/), and secondary (2/sup 0/), and tertiary (3/sup 0/) immunizations with either S. pneumoniae R36a (Pn) or PC coupled to keyhole limpet hemocyanine (PC-KLH). Concentrations of PC-binding activity and of four distinct T15 idiotopes (Id) detectable with monoclonal anti-Id AB1-2, MaId5-4, B36-82 and B24-44 were measured in solid-phase radioimmunoassay. These Id are independent clonotypic markers of the anti-PC antibody repertoire. The serum levels of the Id had kinetics distinct from each other and from the total PC-binding activity. For example, the concentration of AB1-2 decreased after 2/sup 0/ and 3/sup 0/ challenge with PC-KLH while the levels of B36-82 and B24-44 remained stable in several strains. Marked differences in Id serum levels were observed among various strains; however, their genetic mapping has been difficult in that an apparent effect of H-2 locus seemed to be modified by the genetic background of the mice. The only unambiguous linkage was the absence of AB1-2 and MaId5-4 in mice bearing Igh/sup j/. The authors data indicate that idiotopically-distinct T15/sup +/ clones are selected during an antigen-driven response in genetically different mice. Mechanisms regulating these patterns are being studied.
- Research Organization:
- Univ. of Texas Medical Branch, Galveston
- OSTI ID:
- 5326099
- Report Number(s):
- CONF-8604222-; TRN: 86-030450
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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ALCOHOLS
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AMMONIUM COMPOUNDS
ANIMALS
ANTIBODIES
DRUGS
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HYDROXY COMPOUNDS
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QUATERNARY COMPOUNDS
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RADIOIMMUNOLOGY
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550201* - Biochemistry- Tracer Techniques