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Effects of chronic ethanol ingestion on arachidonic acid (AA) metabolism in rat macrophages

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5324575
; ; ; ;

In a previous report, preincubation of rat platelets with ethanol resulted in dose dependent inhibition of AA metabolites whereas chronic ingestion of ethanol enhanced the synthesis of AA metabolites. Thus, the authors studied whether chronic ethanol ingestion also affects AA metabolism in MACS. Two groups of rats (10 each) were fed DeCarli/Lieber liquid diet containing 36 caloric % ethanol for 3 weeks. The control group was pair fed the same diet made isocaloric with dextrin-maltose. Resident MACS were collected by peritoneal lavage. The monolayers of MACS were incubated for 20 min with calcium ionophore (5..mu..g/ml), and the incubation stopped with 4 vol. of ethanol. PGE/sub 2/, LTB/sub 4/ and 5-HETE were assayed by radioimmunoassay. The results indicated that chronic ethanol ingestion did not affect the capacity of MACS to synthesize AA metabolites. There was also no difference in the levels of AA metabolites in heart and lung homogenates between the two groups.

Research Organization:
Louisiana State Univ. Agriculture Center, Baton Rouge
OSTI ID:
5324575
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550501 -- Metabolism-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMAL CELLS
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHRONIC EXPOSURE
CONNECTIVE TISSUE CELLS
ETHANOL
HEART
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INGESTION
INTAKE
ISOTOPE APPLICATIONS
LUNGS
MACROPHAGES
MAMMALS
METABOLISM
METABOLITES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES