Rhesus monkey platelets
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5322880
The purpose of this abstract is to describe the adenine nucleotide metabolism of Rhesus monkey platelets. Nucleotides are labelled with /sup 14/C-adenine and extracted with EDTA-ethanol (EE) and perchlorate (P). Total platelet ATP and ADP (TATP, TADP) is measured in the Holmsen Luciferase assay, and expressed in nanomoles/10/sup 8/ platelets. TR=TATP/TADP. Human platelets release 70% of their TADP, with a ratio of released ATP/ADP of 0.7. Rhesus platelets release 82% of their TADP, with a ratio of released ATP/ADP of 0.33. Thus, monkey platelets contain more ADP than human platelets. Thin layer chromatography of EE gives a metabolic ratio of 11 in human platelets and 10.5 in monkey platelets. Perchlorate extracts metabolic and actin bound ADP. The human and monkey platelets ratios were 5, indicating they contain the same proportion of actin. Thus, the extra ADP contained in monkey platelets is located in the secretory granules.
- Research Organization:
- Univ. of Illinois College of Medicine, Chicago
- OSTI ID:
- 5322880
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENINES
ADP
AMINES
ANIMALS
ANTIMETABOLITES
AROMATICS
ATP
AZAARENES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
DRUGS
EXTRACTION
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MAN
MATERIALS
METABOLISM
MONKEYS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PURINES
QUANTITY RATIO
SECRETION
SEPARATION PROCESSES
SOLVENT EXTRACTION
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ADENINES
ADP
AMINES
ANIMALS
ANTIMETABOLITES
AROMATICS
ATP
AZAARENES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
DRUGS
EXTRACTION
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MAN
MATERIALS
METABOLISM
MONKEYS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PURINES
QUANTITY RATIO
SECRETION
SEPARATION PROCESSES
SOLVENT EXTRACTION
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
VERTEBRATES