Regulation of collagen synthesis in fibroblasts within a three-dimensional collagen gel

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90417-X· OSTI ID:5322200

Mauch, C; Hatamochi, A; Scharffetter, K; Krieg, T ^[1]

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1. Dermatologische Klinik der Ludwig Maximilians Universitaet, Muenchen (West Germany)

Fibroblasts cultivated within a three-dimensional collagen gel display an elongated, spindle-like morphology, reduce their proliferation rate, contract the gel to a very dense tissue, and modify their metabolic activity as compared to monolayer cultures. Collagen synthesis measured as protein-bound hydroxyproline is reduced to 5% of the values found in monolayer culture. The reduction involving type I and type III collagen is due to decreased de novo synthesis and not to enhanced degradation. Dot blot hybridization, Northern blot analysis, and in situ hybridization using collagen I- and III-specific cDNA probes demonstrate that reduced biosynthesis rates are reflected by a marked reduction of pro{alpha}{sub 1} (I), pro{alpha}{sub 2} (I), and pro{alpha}{sub 1} (III) collagen mRNA indicating pretranslational regulation. A similar reduction was observed for actin mRNA whereas levels of tubulin mRNA were similar for fibroblasts in monolayer culture or cultivated within the three-dimensional collagen gels. The data suggest a specific reprogramming of various cellular activities in response to contact with the reconstituted extracellular matrix.

• OSTI ID: 5322200
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 178:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACTIN
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
AZOLES
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CELL PROLIFERATION
COLLAGEN
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DNA
DNA HYBRIDIZATION
FIBROBLASTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
HYDROXY ACIDS
HYDROXYPROLINE
MESSENGER-RNA
MOLECULAR BIOLOGY
MORPHOLOGY
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRROLES
PYRROLIDINES
RECOMBINANT DNA
RNA
SCLEROPROTEINS
SOMATIC CELLS
SYNTHESIS
TISSUES