Source and role of diacylglycerol formed during phagocytosis of opsonized yeast particles and associated respiratory burst in human neutrophils

Journal Article · Fri Jun 28 00:00:00 EDT 1991 · Biochemical and Biophysical Research Communications; (United States)

DOI:https://doi.org/10.1016/0006-291X(91)90630-P· OSTI ID:5317037

Della Bianca, V; Grzeskowiak, M; Lissandrini, D; Rossi, F ^[1]

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1. Univ. of Verona (Italy)

The results presented in this paper demonstrate that in human neutrophils phagocytosis of C3b/bi and IgG-opsonized yeast particles is associated with activation of phospholipase D and that this reaction is the main source of diglycerides. The demonstration is based upon the following findings: (1) the challenge of neutrophils with these opsonized particles was followed by a rapid formation of (3H)alkyl-phosphatidic acid (( 3H)alkyl-PA) and (3H)alkyl-diglyceride (( 3H)alkyl-DG) in cells labeled with (3H)alkyl-lyso-phosphatidylcholine; (2) in the presence of ethanol (3H)alkyl-phosphatidylethanol was formed, and accumulation of (3H)alkyl-PA and (3H)alkyl-DG was depressed; (3) propranolol, by inhibiting the dephosphorylation of (3H)alkyl-PA, completely inhibited the accumulation of (3H)alkyl-DG and depressed by about 75% the formation of diglyceride mass. Evidence is also presented that phagocytosis of C3b/bi and IgG-opsonized yeast particles and associated respiratory burst can take place independently of diglyceride formation and of the activity of this second messenger on protein kinase C. In fact: (a) propranolol while completely inhibited the formation of diglyceride mass did not modify either the phagocytosis or respiratory burst; (b) these two processes were insensitive to staurosporine.

• OSTI ID: 5317037
• Journal Information: Biochemical and Biophysical Research Communications; (United States), Journal Name: Biochemical and Biophysical Research Communications; (United States) Vol. 177:3; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLESTERASES
CHEMISTRY
ENZYMES
ESTERASES
ESTERS
EUMYCOTA
FUNGI
HYDROGEN COMPOUNDS
HYDROLASES
IN VITRO
ISOTOPE APPLICATIONS
LECITHINS
LEUKOCYTES
LIPASES
LIPIDS
MAMMALS
MAN
MATERIALS
MICROORGANISMS
NEUTROPHILS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHAGOCYTOSIS
PHOSPHOLIPIDS
PLANTS
PRIMATES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
YEASTS