Serotoninergic neuronal systems and ethanol sensitivity in long-sleep (LS) and short-sleep (SS) mice

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5316588

French, T A; Weiner, N

LS and SS mice selectively bred for differences in CNS sensitivity to ethanol (EtOH) exhibit markedly different sleep times (LS = 120 min; SS = 13 min) and differ considerably in hypothermic response following EtOH< 4 g/kg i.p. Basal levels of serotonin (5HT), 5-hydroxyindole acetic acid and 5HT turnover (defined as 5-hydroxy-tryptophan accumulation after inhibition of aromatic L-amino acid decarboxylase) in 6 brain regions (hypthalamus (HYP), dorsal raphe (DR), pontine-medullary raphe (PMR), striatum (STR), hippocampus (HIP) and cortex (CTX)) differ minimally between the two lines. 5 HT turnover in STR, HIP, CTX and PMR of either LS or SS mice or in HYP or DR of SS mice was not altered at either 20 or 120 min after EtOH, 4 g/kg. However, in LS mice, 5HT turnover was decreased 23% in HYP at 20 min and 30-34% in HYP and DR at 120 min. LS mice become 1.2 and 2.2/sup 0/ more hypothermic than SS mice at 20 and 120 min after EtOH, 4 g/kg. When approximately equi-effective hypothermic doses of EtOH were administered to LS (3.5 g/kg) and SS (5.0 g/kg) mice, differences in 5HT turnover were still apparent. Pentobarbital administration (65 mg/kg) both decreased brain 5HT turnover 30-60% and produced comparable degrees of hypothermia in LS and SS mice. These data suggest that the apparent increase in sensitivity to inhibition by EtOH of 5HT neurons of HYP and DR of LS mice may contribute to the greater degree of hypothermia induced in this line.

• Research Organization: Univ. of Colorado School of Medicine, Denver
• OSTI ID: 5316588
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism &amp; Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETIC ACID
ALCOHOLS
AMINES
ANESTHETICS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZINES
AZOLES
BARBITURATES
BIOLOGICAL VARIABILITY
BODY
BODY TEMPERATURE
BRAIN
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DRUGS
ETHANOL
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYPNOTICS AND SEDATIVES
HYPOTHERMIA
INDOLES
MAMMALS
MICE
MONOCARBOXYLIC ACIDS
NEMBUTAL
NERVES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PYRIMIDINES
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RODENTS
SENSITIVITY
SEROTONIN
SYMPATHOMIMETICS
TOXICITY
TRYPTAMINES
VERTEBRATES