Efficient expression of the yeast metallothionein gene in Escherichia coli
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:5313463
The yeast metallothionein gene CUP1 was cloned into a bacterial expression system to achieve efficient, controlled expression of the stable, unprocessed protein product. The Escherichia coli-synthesized yeast metallothionein bound copper, cadmium, zinc, indicating that the protein was functional. Furthermore, E. coli cells expressing CUP1 acquired a new, inducible ability to selectively sequester heavy metal ions from the growth medium.
- Research Organization:
- Smith Kline and French Labs., Swedeland, PA
- OSTI ID:
- 5313463
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 170:1; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
CADMIUM
CLONING
COPPER
DNA-CLONING
ELEMENTS
ESCHERICHIA COLI
FUNCTIONS
KINETICS
METALLOPROTEINS
METALLOTHIONEIN
METALS
MICROORGANISMS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
TOLERANCE
TRANSITION ELEMENTS
ZINC
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
CADMIUM
CLONING
COPPER
DNA-CLONING
ELEMENTS
ESCHERICHIA COLI
FUNCTIONS
KINETICS
METALLOPROTEINS
METALLOTHIONEIN
METALS
MICROORGANISMS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
TOLERANCE
TRANSITION ELEMENTS
ZINC