Induction of UV photoproducts and DNA damage by solar simulator UV irradiation

Journal Article · Wed Oct 01 04:00:00 EDT 1997 · Environmental and Molecular Mutagenesis

DOI:https://doi.org/10.1016/S0027-5107(97)83264-2· OSTI ID:530875

Wolfreys, A; Henderson, L ^[1]; Clingen, P ^[2]

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1. Unilever Research, Sharnbrook, Bedford (United Kingdom)
2. Univ. of Sussex, Falmer, Brigthon (United Kingdom)

The recent increased incidence of skin cancer and the depletion of the ozone layer has increased interest in the ultraviolet (UV) component of natural sunlight and its role in the induction of skin cancer. Previous research on UV radiation has concentrated on UVC (254nm) but, as only UVB and UVA are present in natural sunlight, its relevance is unknown. We have investigated the induction of two forms of direct DNA damage - the pyrimidine dimer and the (6-4) photoproduct - in human DNA repair deficient XP-G (Xeroderma pigmentosum group G) lymphoblastoid cells following exposure to simulated sunlight. As exposure to natural sunlight is highly variable, a solar simulator lamp was used which is known to mimic natural sunlight at midday in Central Europe. Cells were irradiated on ice to minimise DNA repair and the relative induction of pyrimidine dimers and (6-4) photoproducts was measured using specific monoclonal antibodies and a computer assisted image analysis system. A time dependent increase in both cyclobutane dimer and (6-4) photoproduct antibody binding sites was seen. The increases in pyrimidine dimer and (6-4) photoproduct antibody binding sites differed to that reported with natural sunlight in the UK but was similar to that seen with a similar solar simulator lamp.

• Sponsoring Organization: USDOE
• OSTI ID: 530875
• Report Number(s): CONF-9704100--
• Journal Information: Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: Suppl.28 Vol. 29; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
DNA
DNA REPAIR
EVALUATION
SOLAR RADIATION
ULTRAVIOLET RADIATION